Digital Twin Based Design and Experimental Validation of a Continuous Peptide Polishing Step

Optimizing or debottlenecking existing production plants is a challenging task. In this case study, an reversed phased chromatography polishing step for peptide purification was optimized with the help of digital twin. The batch depicted digitally general rate model. Model parameter determination and model validation done dedicated experiments. twin then used to identify process variants, especially continuous steps. MCSGP found achieve high purities yield but at cost productivity due column synchronization. An alternative Continuous Twin Column (CTCC) established that eliminates unnecessary waiting times. Ensuring same higher purity compared process, achieved increase 31% 27.6%. Experimental long runs confirmed these results.